Our Systems
SKI LODGE
The DNA sequence (i.e., gene cDNA) can be introduced as a single copy, regulated by five tissue-specific promoters.
-
We generated a single-copy knock-in loci for defined gene expression (SKI LODGE) system to insert any DNA by CRISPR/Cas9 at defined safe harbors in the C. elegans genome. Utilizing a single crRNA guide, which also acts as a Co-CRISPR enrichment marker, any DNA sequence can be introduced as a single copy, regulated by five different tissue-specific promoters.
-
Universal SKI
Any gene (with regulatory sequences) can be cloned into the universal pSKI plasmid by traditional cloning or Gibson Assembly® and then inserted as a single copy into any chromosome.
-
The Universal SKI System uses the pSKI plasmid (with 47 restriction sites) that facilitates the insertion of a gene of interest at specific safe harbor loci on each autosome. The pSKI plasmid contains two synthetic 900-bp homology arms that recombine with the SKI cassettes. This system also uses a single crRNA guide. Overall, the Universal SKI System is highly flexible.
-